The retention volume VR
of an analyte represents the volume of mobile phase necessary to enable its migration throughout the column from the moment of entrance to the moment in which it leaves. To estimate this volume, different methods (direct or indirect) may be used, that depend of the physical state of the mobile phase. On a standard chromatogram with time in abscissa, VR
is calculated from expression 1.16, if the flow rate F is constant,
of an analyte represents the volume of mobile phase necessary to enable its migration throughout the column from the moment of entrance to the moment in which it leaves. To estimate this volume, different methods (direct or indirect) may be used, that depend of the physical state of the mobile phase. On a standard chromatogram with time in abscissa, VR
is calculated from expression 1.16, if the flow rate F is constant,
The volume of a peak, Vpeak
corresponds to that volume of the mobile phase in which the compound is diluted when leaving the column. It is defined by:
corresponds to that volume of the mobile phase in which the compound is diluted when leaving the column. It is defined by:
