The volume of the mobile phase in the column (known as the dead volume), VM
, corresponds to the accessible interstitial volume. It is often calculated from a chromatogram, provided a solute not retained by the stationary phase is present. The dead volume is deduced from tM
and the flow rate F:
, corresponds to the accessible interstitial volume. It is often calculated from a chromatogram, provided a solute not retained by the stationary phase is present. The dead volume is deduced from tM
and the flow rate F:
Sometimes, in the simplest cases, the volume of the stationary phase designated by VScan be calculated by subtracting the dead volume V
from the total internal volume of the empty column.